Journal:

Article Title: IL-1ra and IL-1 production in human oral mucosal epithelial cells in culture: differential modulation by TGF-?1 and IL-4

doi: 10.1046/j.1365-2249.2002.01685.x

Figure Lengend Snippet: Western-blot analysis of IL-1ra protein in lysate and supernatant of oral mucosal cells. Lane 4 is a fraction of a concentrated pool of supernatants from three unstimulated cultures. Cell-associated IL-1ra is shown from unstimulated cells (lane 5). For comparison, lane 3 is the 25 kD monocyte IL-1ra. Lane 2 is the biotinylated standard (mol. wt. × 103 daltons). Lane 1 is the control with preimmune serum.

Article Snippet: The specificity of the reaction was checked by using a preimmune serum (goat serum, Dako, Trappes, France) instead of the specific anti-IL-1ra serum.

Techniques: Western Blot

Journal:

Article Title: IL-1ra and IL-1 production in human oral mucosal epithelial cells in culture: differential modulation by TGF-?1 and IL-4

doi: 10.1046/j.1365-2249.2002.01685.x

Figure Lengend Snippet: Immunohistological staining in scattered oral mucosal cells (×20). These results are representative of two similar experiments. (a) IL-1ra staining is observed in a few unstimulated cells. (b) It is more intense in the cytoplasm of cells stimulated with TGF-β1. (c) These cells stain markedly positive for involucrin. (d) Staining was nil in control with preimmune serum. Control was also negative with anti-IL-1ra serum preincubated with recombinant IL-1ra.

Article Snippet: The specificity of the reaction was checked by using a preimmune serum (goat serum, Dako, Trappes, France) instead of the specific anti-IL-1ra serum.

Techniques: Staining, Recombinant

Journal: Journal of Bacteriology

Article Title: Identification and Characterization of a New Ferric Enterobactin Receptor, CfrB, in Campylobacter ▿ †

doi: 10.1128/JB.00478-10

Figure Lengend Snippet: Critical role of CfrB in Campylobacter-host interaction. (A) Effect of the CfrB mutation on colonization of chickens by C. jejuni JL11 and C. coli JL170. The experiment included two groups, and chickens in each group (15 birds) were inoculated with a 1:1 mixture of a specific Campylobacter strain and its isogenic cfrB mutant. Fecal samples were collected by cloacal swabs and used for CFU enumeration. Each bar represents the mean log transformed CFU of each strain in each group at the indicated days postinoculation (DPI). The broken line indicates the limit of detection. (B and C) Inactivation of CfrB abolishes C. coli JL170 colonization of chickens. The experiment included three groups of birds. The first two groups of birds were inoculated with JL170 or its isogenic cfrB mutant (JL170 CfrB−) (B), while the third group was infected with a 1:1 mixture of the two strains (C). For each group, six birds were euthanized and the cecal contents were collected at the indicated DPI. Each symbol indicates the log number of CFU/g of cecal contents for a single chicken. The horizontal bars indicate the means of groups at the indicated times. The broken line indicates the limit of detection. (D) Inhibitory effect of CfrB-specific antiserum on FeEnt-mediated growth promotion in Campylobacter. CfrB antiserum, MOMP antiserum, preimmune serum, or PBS was mixed with JL11 and JL170 cells for a modified growth promotion assay as described in Materials and Methods.

Article Snippet: Prior to the assay, the CfrB-specific rabbit antiserum, the preimmune control rabbit serum, and the major outer membrane protein (MOMP) rabbit antiserum specific for Campylobacter (kindly provided by Qijing Zhang, Iowa State University) were heat inactivated at 56°C for 30 min. To evaluate the inhibitory effect of anti-CfrB serum on CfrB function, 1 ml of MH broth grown log-phase culture of each Campylobacter strain was mixed with 1 ml of either CfrB antiserum, preimmune serum, MOMP antiserum, or phosphate-buffered saline (PBS) and then added to 23 ml of MH agar containing 20 μM DFO and poured into petri dishes for solidification.

Techniques: Mutagenesis, Transformation Assay, Infection, Modification